Q-PCR Alert Kits – Oncohematology

Product Range
The Reagents are sufficient for 24 duplicate determinations for the rearrangement and 24 duplicate determinations for ABL cDNA.

Each kit is composed of mastermix specific to the a region of BCR-ABL rearrangement, mastermix specific to a region of the ABL human gene, microplates and adhesive sheets.
Catalogue Number(s)
RTSG07-210
Product Description: Philadelphia p210 Q – PCR Alert Kit
Number of Tests: 48 x 2
Methodology:

• Detection of the cDNA BCR-ABL rearrangement variant P210
• Sample type: Whole blood, Leucocytes suspensions, Lymphomonocyte suspensions
• Real-Time platform: ABI 7000 series Real-Time PCR System
• Manual Extraction: EXTRAzol

RTSG07-190
Product Description: Philadelphia p190 Q – PCR Alert Kit
Number of Tests: 48 x 2
Methodology:

• Detection of the cDNA BCR-ABL rearrangement variant P210
• Sample type: Whole blood, Leucocytes suspensions, Lymphomonocyte suspensions
• Real-Time platform: ABI 7000 series Real-Time PCR System
• Manual Extraction: EXTRAzol

TaqMan® is a registered trademark of Roche Molecular Systems, Inc.

Kits are CE-IVD Marked

Legal Notice:
This product is sold under licensing agreement by F. Hoffmann-La Roche AG

Standards
Philadelphia Q – PCR Standards are composed of 4 stabilized plasmid solution at given titers: 105, 104, 103 and 102. Each vial of ready-to-use standard has a color coded cap that refers to the plasmid’s titer.
Catalogue Number(s)
STDG07-210
Product Description: Philadelphia p210 Q – PCR Standard
Number of Tests: 4 x 2

STDG07-210
Product Description: Philadelphia p190 Q – PCR Standard
Number of Tests: 4 x 2

Kits are CE-IVD Marked

ELITech Molecular Diagnostic also offers a Philadelphia p210 RNA reference kit. This kit provides solution of total RNA to assess the quantification by Real-Time PCR assay of the ratio between BCR-ABL and ABL gene. This product allows establishing the theoretical conversion factor. This factor is a unique and essential tool to establish the accuracy of the Real-Time PCR for BCR-ABL assay, to standardize the assay and to correctly evaluate the molecular response during CML patients follow up.

Each solution is obtained by diluting a known quantity of RNA from a positive cell line positive for the t(9;22) translocation (BCR-ABL rearrangement p210-b3a2) with total RNA form a normal cell line. Based on the results obtained with this product, the conversion factor can be calculated. Once the conversion factor is assigned by a reference center, the laboratory can use the Philadelphia p210 RNA reference as a tool to follow up the reliability of the conversion factor.

The product is composed of 4 different concentrations of total RNA package in color codes vials.
Catalogue Number(s)
SPG07-210
Product Description: Philadelphia p210 RNA reference
Number of Tests: 6

CE-IVD marked