MYCOFAST® Screening RevolutioN – Complement MYCOFAST® RevolutioN

Mycoplasmas are the smallest and simplest of the procaryotypes capable of self-reproduction (0.15 to 0.25 μm). They differ from other bacteria in their lack of a cell wall and hence a natural resistance to ß-lactams. Since mycoplasmas are relatively fragile, they will only grow in acellular cultures in the presence of various growth factors and at a constant temperature of 35 to 37 °C.

Most human mycoplasmas are commensal. Of the 9 species that have been isolated from the urogenital tract, Ureaplasma urealyticum and Mycoplasma hominis are the most commonly found. U.u. and M.h. are sexually transmitted and can be pathogenic. Respiratory infections or meningitis can occur in the neonate as a result of contamination from the genital tract at birth. In adults, the infections caused by U.u. and M.h. are described in the table below:

Conventional diagnosis is based upon culture on A7 agar plates followed by microscopical identification of U.u. (sea urchin shaped) or M.h. (fried-egg shaped) colonies.

Since both U.u. and M.h. are commensal, infection can only be diagnosed through the determination of the pathological threshold, followed by precise enumeration.

MYCOFAST® Screening RevolutioN and its COMPLEMENT MYCOFAST® RevolutioN give the solution and make your urogenital diagnosis much easier.

Principle

MYCOFAST® Screening RevolutioN identifies and differentiates U.u. from M.h. after incubating the clinical specimen in 2 wells containing 2 specific and separated growth media.

During growth, U.u. and M.h. metabolize urea and arginine respectively resulting in a color change of the medium, which contains phenol red indicator, from yellow-orange to red. This color change is due to liberation of ammonia resulting in an alkaline pH of the medium.

Mycoplasma growth thus viewed enables:

• Screening of U.u and M.h with the MYCOFAST® Screening RevolutioN. And in case of positivity, complete your diagnosis with the COMPLEMENT MYCOFAST® RevolutioN:
• Enumeration of mycoplasma based on the rate of urea or arginine hydrolysis, which is proportional to the number of germs contained in the sample.
  U.u: > 10³, 10⁴ and 10⁵ UCC/ml
  M.h: > 10⁴ UCC/ml
• Identification based on the sensitivity or otherwise of the germ to three antibiotics (lincomycin, SXT and erythromycin) = Identibiotic.
• U.u and M.h susceptibility testing to 5 antibiotics compliant with new worldwide recommendations:
  Levofloxacin (LVX) 1-2-4 μg/mL , Moxifloxacin (MXF) 0.25-2 μg/mL , Erythromycin (E) 8-16 μg/mL, Clindamycin (CM) 0.25-0.5 μg/mL and Tetracyclin (TE) 1-2-4-8 μg/mL.

Simple methodology

Easy-to-read and easy-to-interpret results

The results are read by the color obtained in the different wells. Urogenital mycoplasma growth is indicated when the medium turns red (alkaline). The medium remains yellow when no growth of urogenital mycoplasma occurs.

Reagents and material

• UMMt:
  Vial of 3 mL mycoplasma broth with antimicrobial agents and preservative solution. pH: 6.0 ± 0.1 25
  REF 00061 UMMtRevolutioN – Quantity: 50
• MYCOFAST® Screening RevolutioN:
  Divisible tray for 10 wells for 5 tests, individually packed in an aluminium sachet with an integrated desiccant
  REF 00063 MYCOFAST^® Screening RevolutioN – Quantity: 10
• Labels:
  Sheet of 5 divisible labels
  REF 00063 MYCOFAST^® Screening RevolutioN – Quantity: 10
• S.M.h:
  vial of 4.5 mL Mycoplama hominis growth activator
  REF 00062 Complement MYCOFAST^® RevolutioN – Quantity: 2
  REF 00063 MYCOFAST^® Screening RevolutioN – Quantity: 1
• MYCOFAST® RevolutioN:
  Tray of 20 wells packed in an aluminium sachet with an integrated desiccant
  REF 00062 Complement MYCOFAST^® RevolutioN – Quantity: 25
• Closing system:
  Protective translucent plastic tray lid
  REF 00062 Complement MYCOFAST^® RevolutioN – Quantity: 25
  

Material required but not provided

• Sample collecting material (Swabs, cytobrushes, sterile containers for liquid samples)
• MYCOPLASMA STABILIZER (REF. 00064)
• Pipettes and tips
• Waste container for contaminated waste
• Mineral oil
• Incubator at 37 ± 1 °C

Stability and storage

• All the reagents are ready-to-use. The vials may be stored at 2-8 °C, in their original packaging until the expiry date shown on the kit.
• Should only one or two, three, or four rows of (U.u) (M.h) wells be used, the remaining MYCOFAST® Screening RevolutioN tray may be stored for 4 weeks at 2-8 °C in its original packaging and hermetically resealed.
• The UMMt medium may be stored temporarily at room temperature but is more stable at
  2-8 °C.
• The S.Mh supplement is stable for 3 months after opening.
• Do not freeze the reagents contained in the kit.