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Test combining identification and antifungal resistance testing of the main pathogenic yeasts.


Intended use

The frequency of fungal infections and in particular those associated with yeasts have increased considerably over the last ten years. The identification of the species, whether as a single agent or of different types, present in a clinical specimen has become indispensable for determining the correct therapeutic option.

The FUNGIFAST® kit allows the identification of the main human pathogenic yeasts as well as the testing of their susceptibility to various antifungal agents used in the treatment of superficial or systemic mycoses.


The FUNGIFAST® kit allows the identification of the following yeasts: Candida albicans, C. guilliermondii, C. lusitaniae, C. parapsilosis, C. kefyr, C. krusei, C. glabrata, C. tropicalis, Cryptococcus neoformans, Saccharomyces cerevisiae.

The identification test is based on:
– Hydrolysis of 2 chromogenic substrates
– Assimilation of 5 carbohydrates
– Hydrolysis of urea
– Detection of the phenoloxydase

This test is combined with an antifungal susceptibility test using:
– Modified RPMI medium with redox indicator
– 5 main dehydrated antifungal agents tested
– 2 concentrations tested for Amphotericin B, Flucytosine, Fluconazole, Itraconazole and 1 concentration tested for Voriconazole

The test is performed using colonies which should be young (24 to 48 hours old) and perfectly isolated on an agar medium in a Petri dish.
All the necessary reagents are contained in the 20-well tray (1 test/tray) and no additional reagent is required to perform the test.

Simple protocol

Easy-to-read and easy-to-interpret results

Results are visualized by a simple spontaneous colorimetric reaction, without instrument or expert interpretation. The identification and resistant test-tray are read by referring to the “color charts” and “result sheet” included in the kit. Identification table & coding systems are available for the interpretation of the results. A detailed table of the critical concentrations (μg/mL) commonly used for the interpretation of the antifungal results is included in the instruction leaflet.

Reagents and Material

    4 mL-vial of buffered semi-agar medium for colony suspension and identification, containing Bacto Agar, colimycin and vancomycin.
    Ref. 44408 Quantity: 10
    Ref. 44430 Quantity: 35
    5 mL-vial of liquid medium (modified RPMI medium) for susceptibility testing, containing resazurin (growth indicator) and glucose.
    Ref. 44408 Quantity: 8
    Ref. 44430 Quantity: 30
    4 mL vial of barium sulphate solution for the control of inoculum standardization.
    Ref. 44408 Quantity: 1
    Ref. 44430 Quantity: 1
    20 well-tray individually packed in an aluminium sachet.
    Ref. 44408 Quantity: 8
    Ref. 44430 Quantity: 30

Stability and Storage

  • The reagents stored at 2-8 °C in their original state are stable until the expiry date indicated on the box.
  • SUSPENSION FUNGI, MES FUNGI as well as the FUNGIFAST® trays are ready-to-use and should be used immediately after opening.


  • The evaluation of the performance characteristics was performed on different yeasts for the most freshly isolated from clinical samples.
  • Identification: The parallel comparative study was carried out with the Biorad AUXACOLOR 2 method. 98.1% strains were correctly identified.
  • Susceptibility testing: The parallel comparative study was carried out with the Biomérieux ATB FUNGUS 3. The overall agreement was 87.5%.

Material required but not provided

  • Paraffin oil
  • Sterile PASTEUR pipettes
  • Incubator at 37 °C
  • Container for contaminated waste

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