The frequency of fungal infections and in particular those associated with yeasts have increased considerably over the last ten years. The identification of the species, whether as a single agent or of different types, present in a clinical specimen has become indispensable for determining the correct therapeutic option.
The FUNGIFAST® kit allows the identification of the main human pathogenic yeasts as well as the testing of their susceptibility to various antifungal agents used in the treatment of superficial or systemic mycoses.
The FUNGIFAST® kit allows the identification of the following yeasts: Candida albicans, C. guilliermondii, C. lusitaniae, C. parapsilosis, C. kefyr, C. krusei, C. glabrata, C. tropicalis, Cryptococcus neoformans, Saccharomyces cerevisiae.
The identification test is based on:
– Hydrolysis of 2 chromogenic substrates
– Assimilation of 5 carbohydrates
– Hydrolysis of urea
– Detection of the phenoloxydase
This test is combined with an antifungal susceptibility test using:
– Modified RPMI medium with redox indicator
– 5 main dehydrated antifungal agents tested
– 2 concentrations tested for Amphotericin B, Flucytosine, Fluconazole, Itraconazole and 1 concentration tested for Voriconazole
The test is performed using colonies which should be young (24 to 48 hours old) and perfectly isolated on an agar medium in a Petri dish.
All the necessary reagents are contained in the 20-well tray (1 test/tray) and no additional reagent is required to perform the test.
Easy-to-read and easy-to-interpret results
Results are visualized by a simple spontaneous colorimetric reaction, without instrument or expert interpretation. The identification and resistant test-tray are read by referring to the “color charts” and “result sheet” included in the kit. Identification table & coding systems are available for the interpretation of the results. A detailed table of the critical concentrations (μg/mL) commonly used for the interpretation of the antifungal results is included in the instruction leaflet.