Candidas are usually commensal yeasts of the digestive and urogenital mucosas. They become pathogenic only when favorable conditions arise in the host.
Studies show that C. glabrata is the second most commonly encountered yeast in humans and its pathogenic nature is no longer disputed: C. glabrata is responsible for 10 to 25% of all the candidemias. Moreover, C. glabrata is generally less sensitive to azole derivatives, with certain isolates from patients treated with fluconazole or ketoconazole appearing to be resistant to virtually all the azole derivatives.
The isolation and rapid identification of C. glabrata is thus essential to determine the correct therapeutic treatment. Traditionally, biochemical tests are used in the identification of C. glabrata. The disadvantage of the carbon auxanogram and the zymogram is that there is a long 24 to 48 hours wait before the tests can be read.
C. glabrata can be identified more quickly with systems based upon the detection of preformed enzymes that use chromogenic substrates or alternatively, that are based upon the assimilation of trehalose. ELIchromglabrata is a rapid C. glabrata identification test based upon the detection of trehalase.