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ELI.H.A Schistosoma

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ELI.H.A Schistosoma

Indirect hemagglutination test for serodiagnosis of bilharziosis.


Intended Use

Bilharziosis or schistosomiasis represent a group of parasitic diseases caused by non-segmented flat worms of the genus Schistosoma.

In the presence of adult worms, immunity is acquired progressively. However schistosome’s eggs are the pathogenic element of the disease. The disease proceeds in three successive phases: contamination – invasion – disease state.

The diagnosis of bilharziosis can be made by eggs identification. The problem is that eggs cannot be detected before the “disease state” phase. Immunological diagnosis, via the detection of antibodies, is thus essential in the initial stages of this disease.

ELITech developed the ELI.H.A Schistosoma, an indirect haemagglutination assay, for the quantitative determination of anti-Schistosoma mansoni, anti-Schistosoma hæmatobium and anti-Schistosoma intercalatum antibodies in the serum.

It is rapid, easy-to-use and easy-to-read with results available within 2 hours.


Sheep red blood cells are sensitized with Schistosoma mansoni antigen.

Diluted serum is mixed with sensitized sheep red blood cells. If anti-Schistosoma antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well.

Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…).

The reaction is carried out in a U-microplate.

Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.

Simple Protocol

A single protocol for all our ELI.H.A kits.

1. First serum dilution
2. Microplate preparation
3. Serial dilution of serum and preparation of the ‘serum control’ well
4. Addition of sensitized RBC and non sensitized RBC
5. Gently mix the plate and let stand 2 hours before reading

Easy-to-read and easy-to-interpret results

Titer Interpretation
Can correspond to a previous or treated infection.
Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
Presumption of an active infection

Reagents and Material

  • R1:
    Vial of 2.4 mL of sensitized red blood cells
    Quantity: 1
  • R2:
    Vial of 1 mL of non-sensitized red blood cells
    Quantity: 1
  • BUF:
    Vial of 55 mL of phosphate buffer pH 7.2
    Quantity: 1
  • R3:
    Vial of 2 mL of adsorbent
    Quantity: 1
  • CONTROL +:
    Vial of 0.2 mL of titrated positive control
    Quantity: 1
  • CONTROL -:
    Vial of 0.2 mL of negative control
    Quantity: 1
    Microplate with a U-bottom
    Quantity: 2
    Special dropper
    Quantity: 2

Stability and Storage

  • The reagents are ready-to-use.
  • All the reagents are stored at 2-8°C. Do not freeze.
  • The results of the stability study indicate that the product has a stability of 24 months from its manufacture.


  • Diagnostic sensitivity: 84.4%
  • Diagnostic specificity: 96.9%

Material required but not provided

  • Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
  • Contaminated waste containers
  • Centrifuge
  • Hemolysis tubes

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