Distomiasis are parasitic diseases caused by trematodes. The location of the adult worms in the host gives rise to the following classifications:
Hepato-biliary distomatosis, infection by Faciola hepatica is characterized by a progressive life-cycle in the human definitive host, which includes a transperitoneal and transhepatic larval migrationary phase. Diagnosis of F. hepatica is primarily immunological during the invasive phase of infection and relies upon the detection of antibodies (phase where there is no faecal egg transmission). Immunological diagnosis is also of great interest during the early disease state phase, because the eggs are only emitted in small quantities.
ELITech developed the ELI.H.A Distoma, an indirect hemagglutination assay, for the quantitative determination of anti- Faciola hepatica antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours and reliable overnight.
Sheep red blood cells are sensitized with Faciola hepatica antigen.
Diluted serum is mixed with sensitized sheep red blood cells. If anti-Faciola hepatica antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well.
Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…).
The reaction is carried out in a U-microplate.
Each kit allows 102 tests to be carried out or 17 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.
Easy-to-Read and Easy-to-Interpret Result :
Non-significant reactionProbable absence of distomiasisRenew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test
Doubtful reactionRenew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test
Significant reaction in favor of progressive distomiasis
Reagents and Material
R1: Vial of 2.2 mL of sensitized red blood cellsR2: Vial of 1mL of non-sensitized red blood cellsBUF: Vial of 55 mL of phosphate buffer pH 7.2R3: Vial of 2 mL of adsorbent
CONTROL +: Vial of 0.2 mL of titrated positive control
CONTROL –: Vial of 0.2 mL of negative control
MICROPLATE : Microplate with a U-bottom
DROPPER: Special dropper
Material Required but Not Supplied :
Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
Contaminated waste containers
Stability and Storage :
The reagents are liquid and ready-to-use.
All the reagents are stored at 2-8°C. Do not freeze.
The results of the stability study indicate that the product has a stability of 24 months at 2-8°C from its manufacture.