Local conditions (preformed cavities, debrided pulmonary abscesses, impaired mucous membranes…);
General conditions (immunosuppression following major surgery or medical procedures: organ transplantation, immunosuppressive therapy, steroids, antibiotics…).
ELITechGroup developed the ELI.H.A Aspergillus, an indirect hemagglutination assay, for the quantitative determination of anti-Aspergillus fumigatus antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours.
Sheep red blood cells are sensitized with Aspergillus fumigatus antigen.
Diluted serum is mixed with sensitized sheep red blood cells. If anti-Aspergillus fumigatus antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well.
Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…).
The reaction is carried out in a U-microplate.
Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.
Easy-to-Read and Easy-to-Interpret Results
Non-significant reactionProbable absence of a deep aspergillosis infection. Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
Doubtful reactionRenew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
Significant reaction in favor of a deep aspergillosis infection
R1: Vial of 2.2 mL of sensitized red blood cellsR2: Vial of 1 mL of non-sensitized red blood cells
BUF: Vial of 55 mL of phosphate buffer pH 7.2
R3: Vial of 2 mL of adsorbent
CONTROL +: Vial of 0.2 mL of titrated positive control
CONTROL –: Vial of 0.2 mL of negative control
MICROPLATE : Microplate with a U-bottom
DROPPER: Special dropper
Materiel required but not supplied
Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
Contaminated waste containers
Stability and storage
The reagents are liquid and ready-to-use.
All the reagents are stored at 2-8°C. Do not freeze.
The results of the stability study indicate that the product has a stability of 16 months at 2-8°C from its manufacture.