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ELI.H.A Aspergillus


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ELI.H.A Aspergillus

Indirect hemagglutination test for serodiagnosis of Aspergillosis.

Description

Intended use

  • Local conditions (preformed cavities, debrided pulmonary abscesses, impaired mucous membranes…);
  • General conditions (immunosuppression following major surgery or medical procedures: organ transplantation, immunosuppressive therapy, steroids, antibiotics…).

ELITechGroup developed the ELI.H.A Aspergillus, an indirect hemagglutination assay, for the quantitative determination of anti-Aspergillus fumigatus antibodies in the serum. It is rapid, easy-to-use and easy-to-read with results available within 2 hours.

Principle
Sheep red blood cells are sensitized with Aspergillus fumigatus antigen.

Diluted serum is mixed with sensitized sheep red blood cells. If anti-Aspergillus fumigatus antibodies are present in the serum, sensitized red blood cells will agglutinate, resulting in a cloudy red/brown deposit coating the well. In the absence of specific antibodies, sensitized red blood cells will not agglutinate, resulting in a ring-like deposit at the bottom of the well.

Non-sensitized red blood cells are also available in the kit. They ensure the specificity of the reaction by allowing identifying any interference from the natural anti-sheep agglutinins (Forssman heteroantibodies, infectious mononucleosis antibodies…).

The reaction is carried out in a U-microplate.

Each kit allows 120 tests to be carried out or 20 reactions of 6 dilutions. Results are obtained within 2 hours and are reliable overnight.

Easy-to-Read and Easy-to-Interpret Results

Title I nterpretation
<1:320 Non-significant reactionProbable absence of a deep aspergillosis infection. Renew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
=1:320 Doubtful reactionRenew the test 2 to 3 weeks later and also carry out an electrosyneresis or an immunoelectrophoresis test.
≥1:640 Significant reaction in favor of a deep aspergillosis infection

Quantity

Description

11

1

1

1

1

2

2

R1: Vial of 2.2 mL of sensitized red blood cellsR2: Vial of 1 mL of non-sensitized red blood cells

BUF: Vial of 55 mL of phosphate buffer pH 7.2

R3: Vial of 2 mL of adsorbent

CONTROL +: Vial of 0.2 mL of titrated positive control

CONTROL –: Vial of 0.2 mL of negative control

MICROPLATE : Microplate with a U-bottom

DROPPER: Special dropper

Materiel required but not supplied

  • Automatic pipette(s) with a pipetting volume adapted to the volume that will be measured
  • Contaminated waste containers
  • Centrifuge
  • Hemolysis tubes

Stability and storage

  • The reagents are liquid and ready-to-use.
  • All the reagents are stored at 2-8°C. Do not freeze.
  • The results of the stability study indicate that the product has a stability of 16 months at 2-8°C from its manufacture.

Performances

  • Diagnostic sensitivity: 80%
  • Diagnostic specificity: 98%

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