The frequency of fungal infections and in particular those associated with yeasts have increased considerably over the last ten years. The identification of the species, whether as a single agent or of different types, present in a clinical specimen has become indispensable for determining the correct therapeutic option.
The CANDIFAST® ES Twin kit enables resistance testing of yeasts to various antifungal agents. The two rows of wells are identical to the resistance test row of the CANDIFAST® tray.
The CANDIFAST® ES Twin kit is an antifungal resistant test adapted to the treatment of superficial mycosis: Amphotericine B, Nystatine, Flucytosine, Econazole, Ketoconazole, Miconazole, Fluconazole.
The test is performed using colonies which should be young (24 to 48 hours old) and perfectly isolated on an agar medium in a Petri dish.
All the necessary reagents are contained in the 20-well tray (1 test/tray) and no additional reagent is required to perform the test.
Pick up an isolated colony then inoculate a vial of Reagent 1 with the colony.
Standardize the inoculum with respect to the Turbidity Control Vial.
Inoculate reagent R2 with 100 μL of inoculated standardized reagent R1.
Dispense into each of the first 8 wells: 100 μL of inoculated Reagent 2 + add 2 drops of paraffin oil.
Easy-to-read and easy-to-interpret results
Results are visualized by a simple spontaneous colorimetric reaction, without instrument or expert interpretation.